Protein Labeling Reagents
Dojindo Molecular Technologies Inc LysoPrime Green (10 μL x 1). High selectivity for lysosomes, high retention even in lysosomes that had their pH neutralized after staining, long-term visualization of lysosomes, Ex=456 nm, Ex=540 nm.
Understanding lysosome-related physiology requires lysosome-specific probes that can track the behavior of lysosomes within cells. As a consequence of being designed simply to target acidic environments, most lysosomal probes have poor specificity towards the lysosome and stain many non-lysosomal acidic compartments. Moreover, existing lysosome probes’ fluorescent intensity is highly reliant on the lysosomes’ pH; their emission intensity is unstable and varies significantly due to differences in acidity among lysosomes, even within a cell. (https://dojindo.com/lms-mapselectionguide/)
Sartorius pHrodo Orange Cell Labeling Kit, Real-Time Phagocytosis Quantification, Compatible With IncuCyte System, 1 Kit
The Sartorius IncuCyte pHrodo Orange Cell Labeling Kit provides a reliable solution for quantifying phagocytosis and efferocytosis using live-cell imaging. It features a unique pH-sensitive fluorophore that offers low fluorescence at neutral pH and a significant increase in fluorescence under acidic conditions. This enables real-time evaluation of cellular behaviors affected by various factors, setting it apart from other assays.
Lumiprobe Cyanine5 NHS ester, 1263093-76-0, 25mg
Molecular formula: C36H42N3BF4O4, Molecular weight: 667.54, Appearance: dark blue solid, Solubility: very poorly soluble in water (0.19 mM = 127 mg/L), good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents; Amine-reactive red emitting fluorescent dye for the labeling of proteins/peptides and amino-oligonucleotides. For the labeling of sensitive proteins, use water-soluble sulfo-Cyanine5 NHS ester.
Lumiprobe THPTA ligand | 760952-88-3 | MFCD27665386 | 100 mg
THPTA ligand | Purity: 95% | Mol Wt: 434.5 | 760952-88-3 | MFCD27665386 | 100 mg
Revvity Health Sciences Inc IVISense DiR 750 Fluorescent Cell Labeling Dye (XenoLight)
IVISense DiR 750 Fluorescent Cell Labeling Dye (XenoLight)
STEMCELL Technologies NeuroFluor™ NeuO, Size: 0.1 mL
NeuroFluor™ NeuO is a membrane-permeable fluorescent probe that selectively labels primary and pluripotent stem cell-derived neurons in live cultures (J C Er et al. Angew Chem Int Ed, 2015). Cells labeled with NeuroFluor™ NeuO can be visualized using fluorescent imaging. Labeling with this probe is non-permanent; it can be washed off, providing unlabeled, viable cells for downstream applications. For additional information, see References.
ACROBiosystems Antibody Internalization Detection Reagent, 100 tests
Antibody Internalization Detection Reagent labels human IgG Fc with a pH-sensitive red fluorescent dye to enable real-time monitoring of antibody endocytosis in live cells. It forms a stable fluorescent complex rapidly and is compatible with flow cytometry and fluorescence imaging.
- Labels human IgG Fc with a pH-sensitive red fluorescent dye.
- Enables real-time monitoring of antibody endocytosis in live cells.
- Compatible with flow cytometry and fluorescence imaging.
- High signal-to-noise ratio with bright fluorescence in acidic compartments.
- Rapid labeling, complete in about 10 minutes.
- Lyophilized for long-term storage; protect from light and avoid repeated freeze-thaw cycles.
LICOR IRDye 800CW NHS Ester (5.0 mg)
IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are used in a variety of NIR imaging applications including Western blotting, plate-based assays, protein arrays, tissue section imaging, and molecular activity measurements. Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.
Lumiprobe Sulfo-Cyanine5 maleimide | 2242791-82-6 | MFCD28385506 | 1 mg
Sulfo-Cyanine5 maleimide | Purity: 95% | Mol Wt: 803 | 2242791-82-6 | MFCD28385506 | 1 mg
Bio Rad Laboratories PROTEIN ASSAY DYE RGNT 450MLP
Bio-Rad Protein Assay Dye Reagent Concentrate, 450mL, A colorimetric assay for measuring total protein concentration, The dye binds to primarily basic and aromatic amino acid residues
LICOR IRDye 680RD BoneTag™ Optical Probe (4 x 10 nmol)
IRDye 680RD BoneTag Optical Probe is a calcium-chelating compound conjugated to IRDye 680RD near-infrared dye. Using IRDye NIR dyes extends fluorescence signal detection to the near-infrared fluorescent region of the spectrum without affecting the compound’s ability to function as a marker of the mineralization process. NIR fluorescence detection improves depth of penetration due to low tissue autofluorescence, translating to low background interference. Calcium-chelating compounds have been used effectively for the detection of bone mineralization, growth, and morphological changes, including tetracycline derivatives, xylenol orange, alizarin, calcein, and fluorescein. NIR-labeled pamidronate has also been successfully used for detection of arterial calcification in non-invasive optical imaging.
LICOR IRDye 800CW Protein Labeling Kits (high molecular weight kit)
IRDye 800CW Protein Labeling Kits can be used to label antibodies and other proteins for applications such as Western blots, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. The high molecular weight kit is optimized for labeling 1.0 mg amounts of protein with molecular weight 45 - 200 kDa. This kit contains 3 x 0.1 mg IRDye 800CW Reactive Dye vials, 0.5 mL 1 M Potassium Phosphate (K2HPO4) (pH 9), 25 mL 1X PBS, 0.5 mL ultra-pure water, 3 x Pierce Zeba™ Desalting Spin Columns, Pierce Zeba Desalting Spin column instructions, and Protocol for IRDye 800CW Protein Labeling Kit - High MW